Waters intellistart tuning. Rainville PD, Plumb RS, Baynham G.
- Waters intellistart tuning For experienced MS users, this is a valuable time-saving tool. These cookies are necessary for the website to function and cannot be switched off in our systems. In addition, the influence of quadrupole resolving power on ion transmission and method robustness are discussed, along with the ability of Intellistart to OBJECTIVE: Perform resolution setup and calibration for a Waters SQD2 mass detector using IntelliStart. Liquid Chromatography. Go to the file and save the spectrum. This application brief demonstrates that with a few simple steps, Waters’ LC-MS instruments, both quadrupoles and TOFs, can be set up and sample schedules run. SYMPTOMS: IntelliStart Instrument Resolution struggles to pass at Unit Mass Resolution and High Mass Resolution IntelliStart Instrument Resolution fails at Low Mass Resolution and Quantitative The SYMPTOMS: IntelliStart resolution and calibration setup was run Mass positions on tune page and/or acquisition are off more than expected CAUSE: The Tune page for customers sample was not updated Mass position is off after IntelliStart resolution and calibration setup - WKB84960 - Waters Mass Spectrometry (Xevo TQ-S) standards kit for system assessment. Wait for the tray to dry. Learn more: Waters™ Tandem Quadrupole MS systems are equipped with Intellistart Software, which facilitates the fully automated development of robust, high sensitivity MRM methods. OBJECTIVE: Enter a deuterated compound in the Molecular Mass / Formula field in IntelliStart Sample Tune and Develop Method. They are usually only set in response to actions made by you which amount to a request for services, such as setting your privacy preferences, logging in or filling in forms. There are no recommended articles. PROCEDURE: In the Molecular Mass / We thoroughly cleaned the source, cone, block, and Stepwave optics, then pumped down for 48 hours. When developing an MS method, the IntelliStart software enables you to develop LC-MS methods automatically. removing from service troubleshooting with Connections Insight ZSpray source tuning turbomolecular pump 1-18 T-Wave/ScanWave collision cell 1-12 Universal sprayer, The power of mass spectrometry comes easier with IntelliStart from Waters. Open the console and go to Xevo TQD > IntelliStart. Browse to the C:\MassLynx\Instrument_Folder Waters™ Tandem Quadrupole MS systems are equipped with Intellistart Software, which facilitates the fully automated development of robust, high sensitivity MRM methods. View and Download Waters Xevo TQ-XS Mass overview and maintenance manual online. Stuff a Kimwipe or the like into the tray so it catches drips. 2 Components: 1. In Empower, IntelliStart calibration records are saved in each project, not in a global default location as in MassLynx. Load Uncal as your calibration curve in IntelliStart. Waters Brands. Making UPLC-MS Accessible Through MassLynx Software | Waters Liquid Chromatography. Ensure that the resolution mode is calibrated and the tune page has the correct Liquid Chromatography. Collect continuum data in the polarity and mode that need calibration. Waters™ Tandem Quadrupole MS systems are equipped with Intellistart Software, which facilitates the fully automated development of robust, high sensitivity MRM methods. IntelliStart Waters and provides optimum tuning for your samples. Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. In the IntelliStart setup parameter Sample Tune and Develop Method, what value is entered into the Parent Mass column when Multiply Charged Parents is enabled? - WKB28162 Expand/collapse global location This application brief demonstrates that with a few simple steps, Waters’ LC-MS instruments, both quadrupoles and TOFs, can be set up and sample schedules run. For TQ Detector specifications, see the Waters TQ Detector Site Preparation Guide. Open the console and go to the IntelliStart section under the MS. About Press Copyright Contact us Creators Advertise Developers Terms Privacy Policy & Safety How YouTube works Test new features NFL Sunday Ticket Press Copyright Waters™ Tandem Quadrupole MS systems are equipped with Intellistart Software, which facilitates the fully automated development of robust, high sensitivity MRM methods. ERA In this application note, we describe the use of innovative software, Waters IntelliStart, and hardware tools, the ACQUITY TQD tandem mass spectrometer, to rapidly develop an MRM method for the non-steroidal anti-inflammatory drug (NSAID) ibuprofen with subsequent incorporation into an LC-MS/MS method for separation, detection, and quantitation. Select either Quantitative or Low Resolution for the resolution mode. STRATEGY. Setting up with IntelliStart IntelliStart instrument resolution setup fails on Vion, Xevo TQ-S micro, Xevo TQ-S cronos or Xevo TQ-XS - WKB205444 Expand/collapse global location Waters is aware of ongoing quality issues with the raw Ultramark material that ERA uses to make the setup solutions. This is useful for new users, but also saves valuable time FIX or WORKAROUND. 11 Components: 1. Once the tuning has successfully completed, a report is generated, and the conditions can be automatically imported into an MS method. We can get Intellistart to tune "resolution" and "calibration" files in IntelliStart automatically determines MRM transition (s) for each compound and will optimize the MSMS parameters to create an MS method file. IntelliStart will automatically: Monitor system parameters Perform mass calibration and set resolution Tune source conditions to each compound Generate an MS Method for SIR or MRM Check LC-MS system performance Enable automated generation of optimized SIR and/or MRM methods for new compounds Tuning can be carried out in “combined infusion” mode to optimize the method at an appropriate LC flow rate Rainville PD, Plumb RS, Baynham G. 6 Running the mass spectrometer at high flow rates IntelliStart technology. Title: Xevo TQ-S Quick Reference Card, 715002205 Revision A Author: Waters Technical Communications Department Subject: Xevo TQ-S Created Date: 8/12/2010 2:24:50 PM Basic tuning and calibration familiarization for new LCMS TOF users. id6920, TQD, TQDIVD IntelliStart Sample Tune and Develop Method (STDM) fails to find parent ion, even though a strong signal is seen in the Tune page and with manual acquisition from the Tune page. Mass Spectrometry (Xevo TQ-S) standards kit for system assessment. Automated System Check for Bioanalytical Assays: IntelliStart. Two vial positions are provided to contain calibrants and samples for tuning which are automatically metered into the mass detector when needed Software Systems Waters Software MassLynx 4. 1 x For new users it makes compound tuning and optimization a simple task and reproducible from one operator to another. 1 x 30mL SYMPTOMS: Little or no signal at all during infusion Isolation valve is open Little or no spray from capillary CAUSE: The syringe pump was not purged correctly. OBJECTIVE: Improve speed and reliability when calibrating QTof systems over an extended mass range 100 m/z to 4000-5000 m/z using the Assisted IntelliStart Calibration procedure. Setting up with IntelliStart ADDITIONAL INFORMATION. These include Resolution Setup, m/z Calibration, Sample Tune, and the LC/MS System Check. Adjust the tune page settings to have the same LM resolution, HM resolution, and ion energy as the settings in the calibration file. . Combine the data and use automatic peak detection to center the data. IntelliStart’s user-friendly interface automates routine tasks and allows scientists of all levels to quickly and Waters™ Tandem Quadrupole MS systems are equipped with Intellistart Software, which facilitates the fully automated development of robust, high sensitivity MRM methods. The program uses wizards to provide simple and intuitive workflows that are consistent across all tasks on quadrupole instruments and Time-of-Flight (TOF) instruments. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Tuning can be carried out in “combined infusion” mode to optimize the method at an appropriate LC flow rate Rainville PD, Plumb RS, Baynham G. The power of mass spectrometry comes easier with IntelliStart from Waters. Plumb Waters Corporation Abstract High sensitivity bioanalytical methods are used throughout the drug discovery and development process to provide accurate quantification data to support DMPK, toxicokinetics (TK) studies, and randomized clinical trials. CAUSE. 2008; 720002620EN. 1 x 30mL Calibration Solution: Ortho-Phosphoric Acid 0. This module covers set up of the Xevo G2-XS QTOF mass spectrometer using IntelliStart; includes detector set up, calibration and lockspray set up. 1% Acetonitrle/Water (1:1 V/V) in Clear Nalgene Bottle 2. ERA Tuning can be carried out in “combined infusion” mode to optimize the method at an appropriate LC flow rate Rainville PD, Plumb RS, Baynham G. Remove the leak sensor. PROCEDURE: Tune for Expand/collapse global hierarchy Home Informatics MassLynx IntelliStart Sample Tune and Develop does not start; it says "initializing fluidics" but then nothing happens on SQD2, TQD, Xevo TQ MS, Xevo TQD, Xevo TQ-S, Xevo TQ-S micro or Xevo TQ-XS - WKB25998 This application brief demonstrates that with a few simple steps, Waters’ LC-MS instruments, both quadrupoles and TOFs, can be set up and sample schedules run. Setting up with IntelliStart We can get Intellistart to tune "resolution" and "calibration" files in ESI+ mode, but it repeatedly fails to complete in ESI- mode, complaining about the 1925. Select the most recent . Waters is the SQ Detector parameters were set up via the IntelliStart Software and using the on-line fluidics. You can now achive consistent, quality data in less time. Waters IntelliStart Software provides a simple and automated system check procedure to ensure that a Waters LC-MS system is operating properly. 1 or Empower 2* IntelliStart IntelliStart is a feature within Waters MassLynx Software to aid calibrating the instrument, checking the system, and method development. OBJECTIVE: MRM conditions are not created successfully when using IntelliStart PROCEDURE: Open the file you actually use on the Tune page. Mass Spectrometry (Xevo TQ-S) standard for system assessment. Basic tuning and calibration familiarization for new LCMS TOF users. FIX: Verify that the wash PEEK tube is Basic tuning and calibration familiarization for new LCMS tandem quadrupole users. CAUSE: Weak signal on one of the peaks is forcing the Xevo G2-XS calibration taking too long - WKB91505 - Waters Learn how to tune a Waters tandem quadrupole mass spectrometer in order tooptimize or re-optimize the detection of specific compounds. SYMPTOMS: Source pressure test leak warning message Source pressure test failed message CAUSE: Loose or poorly fitted exhaust tubing between instrument and trap bottle Loose or poorly fitted fitting ( Liquid Chromatography. Processes The power of mass spectrometry comes easier with IntelliStart from Waters. Browse for uncal and click OK. SYMPTOMS: IntelliStart fails to find daughter ion CAUSE: IntelliStart is set to exclude any masses below 100 AMU. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. I tried to touch up the tuning manually until it looks fairly good visually before launching Intellistart, but it seems to ignore my starting settings. Setting up with IntelliStart Waters IntelliStart Software provides a simple and automated system check procedure to ensure that a Waters LC-MS system is operating properly. For new users it makes compound tuning and optimization a simple task and reproducible from one operator to another. Processes such as tuning and calibration can be performed by the click of a button using the on-line fluidics. PROCEDURE: Perform instrument resolution setup and calibration for unit mass Resolution mode in This video describes how to run IntelliStart in the Xevo TQ-GC. 6 ion. 40 2. IntelliStart fails calibration; insufficient signal on Xevo Product Line - WKB19438 - Waters Skip to main content This eLearning module introduces the user to the powerful, automated capabilities of Intellistart for time-of-flight LC/MS systems. How to run detector setup on a Xevo G2-XS QTof - WKB24981 - Waters For new users it makes compound tuning and optimization a simple task and reproducible from one operator to another. 1 x 25mL Xevo TQ-S Tuning Solution X100: Acetominophen (4-Acetamidophenol) 2 ng/ul Caffeine, 2 ng/ul, Sulfadimethoxine, 1 ng/ul, Verapamil, 500 pg/ul, Chloramphenicol, 500 pg/ul, 17-A-Hydroxyprogesterone, 50 ng/ul, 90% Tuning can be carried out in “combined infusion” mode to optimize the method at an appropriate LC flow rate Rainville PD, Plumb RS, Baynham G. Low sensitivity with Tof instrument - WKB65010 - Waters SYMPTOMS: When calibrating the Xevo G2-XS via IntelliStart, the software collects calibration data for longer than expected (>5 minutes). 1 x 30mL Intellistart Resolution Solution X20: Sodium Iodide 2 µg/ul , Cesium Iodide50 ng/ul, Methyl Sulfate Sodium Salt 20 ng/µl in IPA/Water (1:1 V/V) in Clear Nalgene Bottle Waters™ Tandem Quadrupole MS systems are equipped with Intellistart Software, which facilitates the fully automated development of robust, high sensitivity MRM methods. Up to four compounds can be run at a time. In Waters documents, Page 181 specifications calibrating starting case, cleaning 4-15 tuning emergency shutdown vacuum connections external connections verifying state of external wiring Instrument Console flow rates IntelliStart Fluidics system guidelines components ion optics infusion pump, purging leaving ready for operation installing operate LED low-volume vials overview reservoir bottles The SQ Detector parameters were set up via the IntelliStart Software and using the on-line fluidics. This module covers set up of the Xevo TQ-S mass spectrometer using IntelliStart; includes basic tuning, IntelliStart will automatically: Monitor system parameters; Perform mass calibration and set resolution; Tune source conditions to each compound; Generate an MS Method for SIR or MRM; Check LC-MS system performance; Learn how to tune a Waters tandem quadrupole mass spectrometer in order tooptimize or re-optimize the detection of specific compounds. Waters TQ Detector: TP02592 Waters TQ Detector Overview Page 17 IntelliStart enables simplified set-up of the system for use in routine analytical Basic tuning and calibration familiarization for new LCMS TOF users. Article type Guide Audience External Language en-US Released Yes Translated No; Tags. TROUBLESHOOTING CALIBRATION FAILURES: Ensure that the correct peaks are being selected for calibration - WKB7760 Manually clear the peaks causing poor calibration - WKB48139 Ensure that the calibrant bottle is at position C - WKB16264 Adjust your calibration mass range, if possible, to exclude high background peaks - WKB61788 Set the RF settings Basic tuning and calibration familiarization for new LCMS TOF users. See How to clean the cone on a Waters Mass Spectrometer. Liquid transitions and conditions chosen for nitrosamine analysis were optimized using MassLynx IntelliStart Software and These cookies are necessary for the website to function and cannot be switched off in our systems. Note: If the system needs to be run before it's completely dry, the check can be disabled in the MS Console under Intergrated IntelliStart Fluidics Fluidics automation of tuning, calibration, and system QC with IntelliStart control and diagnostics. This module covers set up of the Xevo TQ-S mass spectrometer using IntelliStart; includes basic tuning, MRM method and calibration. Basic tuning and calibration familiarization for new LCMS tandem quadrupole users. IntelliStart monitors the performance of the mass spectrometer and will initiate corrective action if any problems are diagnosed. IntelliStart continually monitors your LC/MS system, providing routine performance checks and resolving any system alerts. Turn on the API Gas and Operate and make the spectra visible How-to-Series from APAC: In this series, our application experts share some of their expertise to help users get the best out of Waters equipment. Select Detector Setup and PROCEDURE: Select the mode (Res/Sens) you are mostly using in your analysis. How to perform resolution setup and calibration for a Waters SQD2 mass detector using IntelliStart - WKB100031; Was this article helpful? Yes; No; Recommended articles. Figure 2. Workflow for the development of a peptide MRM method. Making UPLC-MS Accessible Through MassLynx Software | Waters IntelliStart IntelliStart is a feature within Waters MassLynx Software to aid calibrating the instrument, checking the system, and method development. SYMPTOMS: Instrument goes to Standby mode when using Sample Tune and Method Development The inlet method OK indicator light is red CAUSE: The inlet is not ready, and the shutdown method is configured Liquid Chromatography. 1 x 30mL IntelliStart IntelliStart is a feature within Waters MassLynx Software to aid calibrating the instrument, checking the system, and method development. ACQUITY UPLC System 720003254, October 2009. In addition, the module covers Intellistart operations which monitor mass measurement accuracy and optimal LC/MS system performance. 5 Tuning and calibration information . United States. 1 x 25mL Xevo TQ-S Tuning Solution X100: Acetominophen (4-Acetamidophenol) 2 ng/ul Caffeine, 2 ng/ul, Sulfadimethoxine, 1 ng/ul, Verapamil, 500 pg/ul, Chloramphenicol, 500 pg/ul, 17-A-Hydroxyprogesterone, 50 ng/ul, 90% MeOH:10% Water Flame Sealed Ampule <br> 2. IntelliStart IntelliStart is a feature within Waters MassLynx Software to aid calibrating the instrument, checking the system, and method development. Turkey. ERA Basic tuning and calibration familiarization for new LCMS TOF users. Learn more: http://ww SYMPTOMS: When trying to do a Sample Tune and Develop method in IntelliStart, the system doesn't use the tune page specified Prior to running, can see optimized signals on In this application note, we describe the use of Waters' innovative IntelliStart Software and its use with the ACQUITY UPLC and Xevo TQ MS systems to rapidly develop an MRM method for The power of mass spectrometry comes easier with IntelliStart from Waters. ERA SYMPTOMS: IntelliStart fails calibration CAUSE: Expired calibration solution FIX: Use fresh sample. Waters is the Basic tuning and calibration familiarization for new LCMS TOF users. SYMPTOMS: The tune page closes or crashes unexpectedly while running detector setup in Intellistart CAUSE: Corrupted non-storage files FIX: Exit MassLynx. Use higher flow rate - WKB13748 Ensure that orange fluidics tubing is fully submerged in the wash solution - WKB69940 Most Likely: Purge reference fluidics (at least 5 times) - WKB92145 Tighten PEEK nut at Basic tuning and calibration familiarization for new LCMS tandem quadrupole users. id6836, MALDIG2SI, MLYNX, MLYNXV41, SUPMM, SUPUNIFI, SYNAPTXS, SYNG2HD, SYNG2HDMAL, SYNG2MS, SYNG2MSMAL, SYNG2SHD, SYNG2SHDML, Share How-to-Series: Select the Appropriate Resolution of a Quadrupole by Intellistart on X; Share How-to-Series: Select the Appropriate Resolution of a Quadrupole by Intellistart on LinkedIn; Email How-to-Series: Select the Appropriate Resolution of a Quadrupole by Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. After purchase of the pre-recorded on-line course, an email will be sent confirming the purchase. When the resolution is close to or better than the resolution you recorded in step 2, go to the console and select Normal mode (Intellistart > Configure > Normal mode), select Use calibration profile, and then click Start. 40 2. Intellistart optimizes ionization polarity, precursor ion, product ion(s), source voltages, and collision energies to identify the most optimal acquisition parameters. The selector valve systematically makes connections between the fluidics components to carry out the operations processed by the software. In IntelliStart, click create calibration. Learn more: https://www Mass Spectrometry (Xevo TQ-S) standards kit for system assessment. These include optimizing the detector voltage, calibrating the m/z axis, and setting up the lockspray conditions. cal file. FIX: Change the daughter ion exclusion mass to a value lower than the possible IntelliStart Sample Tune and Develop Method (STDM) fails to find daughter ion, even though a strong signal is seen in the Tune page and with manual SYMPTOMS: Poor sensitivity for sample and lockspray Unable to improve sensitivity with tuning CAUSE: Detector voltage not optimized FIX: Complete detector setup in IntelliStart. Waters is the IntelliStart Fluidics system System operation The software automatically controls solvent and sample delivery during auto-tuning, auto-calibration, and method development. Featured Products. Setting up with IntelliStart SQ Detector parameters were set up via the IntelliStart Software and using the on-line fluidics. This is useful for new users, but also saves valuable time IntelliStart’s Sample Tuning and Develop Method can tune and optimize a mixture of up to four compounds in a single operation. Manual infusion and adjustment of the cone voltage on the Tune page is a third option. Bioanalysis MRM Method Using Intellistart™ and Radar Technology Robert S. Select Browse and browse to C:\MassLynx\Intellistart\Results\ (the desired resolution mode). Mass Spectrometry. This is useful for new users, but also saves valuable time approach as the rest of IntelliStart. The molecular mass values SYMPTOMS: The parent ion is visible on the Tune page IntelliStart fails to complete the sample tune and development weak or no signal CAUSE: The sensitivity of the Basic tuning and calibration familiarization for new LCMS tandem quadrupole users. IntelliStart’s intuitive and easy-to-use software makes Waters LC-MS systems accessible to a wide variety of users. Learn more: https://www Basic tuning and calibration familiarization for new LCMS TOF users. This is useful for new users, but also saves valuable time Using IntelliStart sample tune and development is another option. SYMPTOMS: Cone Voltage Optimization plots in the IntelliStart method development report are very different for the Xevo TQD and the Xevo TQ-S micro Both plot response versus cone voltage from 2 V to Basic tuning and calibration familiarization for new LCMS TOF users. Gostaríamos de exibir a descriçãoaqui, mas o site que você está não nos permite. SYMPTOMS: Calibrations stuck at "creating calibration" in IntelliStart, do not complete CAUSE: Large number of calibration profiles present (>450) FIX: Close MassLynx. Go back to System > Acquisition settings on the Tune page, click Calculate, and set the Veff. IntelliStart’s Sample Tuning and Develop Method can tune and optimize a mixture of up to four compounds in a single operation. 1 x 25mL Xevo TQ-S Tuning Solution X100: Acetominophen (4-Acetamidophenol) 2 ng/ul Caffeine, 2 ng/ul, Sulfadimethoxine, 1 ng/ul, Verapamil, 500 pg/ul, Chloramphenicol, 500 pg/ul, 17-A-Hydroxyprogesterone, 50 ng/ul, 90% MeOH:10% Water Flame Sealed Ampule 2. See IntelliStart fails the calibration on a QTof (Xevo or Synapt) for how to check or improve the mass spectral resolution. Delete unnecessary Calibrations stuck at "creating calibration" in IntelliStart and does not complete using the Xevo G2-XS - WKB229156 - Waters Page 21 Waters Xevo TQ-S: IntelliStart technology IntelliStart™ technology monitors LC/MS/MS performance and reports when the mass spectrometer is ready for use. Choose or create a calibration profile that has manual calibration SYMPTOMS: Failure to create a calibration greater than m/z 2500 with sodium iodide (NaI) solution CAUSE: The NaI solution is old, requires vortex mixing after storage, or the concentration is less FIX or WORKAROUND. Prior to developing IntelliStart methods, instrument resolution and calibration should This eLearning module introduces the user to the powerful, automated capabilities of Intellistart for quadrupole LC/MS systems. PROCEDURE. 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